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KMID : 0613820110210081156
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Journal of Life Science
2011 Volume.21 No. 8 p.1156 ~ p.1162
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Characteristics on Equine Herpesvirus Type 3 from Korea
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Yang Jae-Hyuk
Lim Yoon-Kyu
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Abstract
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Equine coital exanthema caused by equine herpesvirus type 3 (EHV-3) is a venereal disease which seriously drops horse reproduction rates. Here, we isolated EHV-3 from infected horses and investigated their biological characteristics. Initial cytopathic effects such as rounding of cells were detected 48 hours post infection of the virus into RK-13 cells. The infected cells were going to detach from the surface of culture flasks 72 hours post infection. The type of isolated viruses from swabbed samples was EHV-3 by PCR analysis. Glycoprotein G (gG) of isolated EHV-3 has a 99.25 percent similarity rate to that of EHV-3 334/74 control strain. The isolated EHV-3 was named Georo strain. Georo strain consisted of four major proteins including 145 kD, 60 kD, 45 kD and 40 kD, as shown by SDS-PAGE analysis. We hope the newly isolated Georo strain of EHV-3 can be used for studying various aspects of Korean equine coital exanthema.
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KEYWORD
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Characteristics, equine coital exanthema, equine herpesvirus type 3, Korea
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